• Human iPSC Sensory Neurons for HSV-1 Latency

  2026-09-22

  Oh et al. developed a scalable human iPSC-derived sensory neuron system that supports HSV-1 latency and stimulus-induced reactivation. The model combines neuronal functional validation with virological, transcriptional, and chromatin-based criteria, providing a human-relevant platform for studying latency mechanisms and candidate interventions.

• Bradford Protein Assay Kit: Practical Guide

  2026-09-22

  The Bradford Protein Assay Kit provides a rapid protein quantification assay for soluble samples when limited volume, fast turnaround, and routine concentration measurement are priorities. It is best used with compatible aqueous matrices and should be validated or replaced for detergent-rich, strongly colored, turbid, compositionally unusual, or out-of-range samples.

• HRP Goat Anti-Mouse IgG (H+L): K1221 Guide

  2026-09-21

  Affinity-Purified Goat Anti-Mouse IgG (H+L), HRP Conjugated, is a polyclonal Horseradish Peroxidase conjugated secondary antibody for detecting mouse IgG in Western blotting, ELISA, IHC, and ICC. K1221 combines affinity purification with HRP-based signal generation, while its defined PBS, BSA, glycerol, preservative, and temperature-storage conditions support reproducible assay setup.

• Linoleic Acid–PPARα–TF Axis in pLELC

  2026-09-21

  This reference study uses serum proteomics, untargeted metabolomics, and patient-derived xenograft validation to identify a linoleic acid–PPARα–tissue factor axis in primary pulmonary lymphoepithelioma-like carcinoma. Its findings connect lipid-related metabolic changes with tumor-microenvironment remodeling and suggest tissue factor as a candidate therapeutic vulnerability, while the small retrospective cohort requires further validation.

• LLY-507: Designing Better SMYD2 Inhibition Assays

  2026-09-20

  LLY-507 is a selective SMYD2 inhibitor for connecting methyltransferase target engagement with cancer and renal-injury phenotypes. This assay-focused guide explains how to interpret p53 methylation, proliferation, inflammation, and fibrosis readouts without overextending preclinical evidence.

• WY-14643: PPARα at the Cancer–Metabolism Interface

  2026-09-19

  WY-14643, also known as Pirinixic Acid, is a selective PPARα probe for connecting lipid metabolism regulation with inflammatory and tumor-microenvironment biology. This article interprets recent multiomics evidence and translates it into practical assay decisions without treating metabolic findings as direct cancer therapy claims.

• BIBR 1532: Reading Telomerase Inhibition Correctly

  2026-09-19

  BIBR 1532 is a selective telomerase inhibitor for dissecting hTERT-dependent cancer biology. This article presents an assay-centered framework that separates immediate telomerase suppression from delayed telomere attrition, using insights from a recent CF10–EdU study to improve experimental interpretation.

• 20-HETE, TRPV1, and Allokinesis in Dermatitis

  2026-09-18

  A 2024 Theranostics study identifies a peripheral mechanism by which chronic dermatitis blurs itch and pain: elevated 20-HETE activates sensitized TRPV1 channels on MrgprA3-positive sensory neurons. The work combines behavioral, chemogenetic, electrophysiological, imaging, metabolomic, and pharmacological approaches to connect lipid signaling with allokinesis and chronic itch.

• GST-Driven Lambda-Cyhalothrin Resistance in M. usitatus

  2026-09-17

  The reference study provides functional evidence that glutathione S-transferase strengthens lambda-cyhalothrin resistance in Megalurothrips usitatus by supporting antioxidant defenses during pesticide-induced stress. Its combination of MuGSTs1 expression analysis, pharmacological GST inhibition, redox measurements, apoptosis endpoints, and insecticide-sensitivity testing helps distinguish a resistance-associated correlation from a mechanistically relevant defense response.

• METTL14–SMN Interaction Controls m6A Homeostasis

  2026-09-17

  The reference study identifies SMN as an arginine methylation-dependent interaction partner of METTL14, connecting a post-translational modification pathway with RNA m6A regulation. Its cellular and mouse-model evidence links disrupted METTL14–SMN signaling to DNA-repair mRNA methylation, disease-associated fibroblast stress responses, embryonic viability, and hematopoiesis.

• EdU Flow Cytometry Assay Kits (Cy5): Practical Guide

  2026-09-17

  EdU Flow Cytometry Assay Kits (Cy5) provide a non-denaturing approach for measuring DNA synthesis during S phase by combining EdU incorporation with copper-catalyzed click chemistry and Cy5 fluorescence. This guide covers setup, controls, acquisition, and troubleshooting; the assay should not be treated as a standalone measure of total proliferation or used without compatibility checks for copper chemistry and cell-specific EdU incorporation.

• Protease Inhibitor Cocktail: EDTA-Free Workflow

  2026-09-16

  This EDTA-free Protease Inhibitor Cocktail helps limit proteolysis during protein extraction and related assays while avoiding intentional chelation of divalent cations. It is suitable for protease-sensitive workflows such as Western blotting and co-immunoprecipitation, but it should not be treated as a substitute for phosphatase inhibitors or as a guarantee against all sample degradation.

• Human iPSC Sensory Neurons Model HSV-1 Latency

  2026-09-15

  Oh et al. establish a scalable human induced pluripotent stem cell-derived sensory neuron system that supports HSV-1 latency and experimentally induced reactivation. The model combines neuronal functional validation with virological, transcriptional, and chromatin-based latency criteria, creating a human-relevant platform for mechanism and therapeutic studies.

• Cycloheximide in Ribosomal Recoding Assays

  2026-09-15

  Cycloheximide is a protein biosynthesis inhibitor that can reveal how translation dependence shapes viral and cellular phenotypes. This article explains how to use it as a controlled perturbation without confusing global elongation arrest with selective effects on programmed ribosomal frameshifting.

• How p38α Inhibitors Promote WIP1 Dephosphorylation

  2026-09-14

  The preprint Dual-Action Kinase Inhibitors Influence p38α MAP Kinase Dephosphorylation identifies inhibitors that both block p38α activity and increase WIP1-mediated removal of the activation-loop phosphate. Biochemical assays and X-ray structures link this effect to an inhibitor-stabilized activation-loop conformation that exposes phosphothreonine, suggesting a route to more durable and selective kinase inhibition.

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