• Whereas continuous exposure to all three factors resulted in

  2018-11-08

  

  Whereas continuous exposure to all three factors (4+) resulted in higher levels of DE genes, there was no significant difference in the proportion of Foxa2+Sox17+ dhpg when compared to transient Wnt activation (2+2−). Surprisingly, T expression in a fraction of differentiated cells was detected in

• apomorphine br H AZ lysine methylation The lysine methyltran

  2018-11-08

  

  H2AZ lysine methylation The lysine methyltransferases SETD6 and SET7 efficiently methylate H2AZ in vitro (Binda et al., 2013). In particular, a detailed study clearly identified lysine 7 as the preferred site of methylation by SETD6 on H2AZ. This result was confirmed by mass spectrometric analysi

• The goals of this study were to evaluate

  2018-11-08

  

  The goals of this study were to evaluate if human fetal gallbladder contains a clonogenic candidate stem cell population and compare its phenotypic and expression profiles to those of fetal IHBD cells. The evaluation of human fetal gallbladder stem Digoxigenin-11-dUTP would have important ramificati

• br Resource details To generate the human iPSC line N

  2018-11-08

  

  Resource details To generate the human iPSC line, N44SV.5, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, cMyc, Klf4 have been employed (Takahashi et al., 2007). For this purpose, commercially available fibroblasts from a healthy juvenile donor were acquired in

• Using similar approach additional mESC lines could be derive

  2018-11-08

  

  Using similar approach, additional mESC lines could be derived using a Pitx3::GFP reporter mouse (Zhao et al., 2004) and another aSYN mouse model of Parkinson\'s disease, expressing human WT SNCA (Westerlund et al., 2008) (data now shown). These mESC lines (Table 1) can be used to study human SNCA-

• br Experimental Procedures br Acknowledgments We

  2018-11-08

  

  Experimental Procedures Acknowledgments We thank A. Voigt, N. Perrimon, S.X. Hou, X. Zeng, H. Sun, G.H. Beag, and X. Yang for reagents; the Iowa Developmental Studies Hybridoma Bank for antibodies; the Bloomington Stock Center and Drosophila Genetic Resource Center for Drosophila stocks; J.M.

• guanylate cyclase Our studies show that only a subpopulatio

  2018-11-08

  

  Our studies show that only a subpopulation of hESCs at the top of the hierarchy possesses an extensive capacity for self-renewal. Polanco et al. recently showed that guanylate cyclase in this compartment could reestablish the continuum of cell states seen in unfractionated cultures (Polanco et al.

• Prolonged APs and EADs are typical of pharmacologically indu

  2018-11-08

  

  Prolonged APs and EADs are typical of pharmacologically induced LQTS modeled in nonhuman hearts (Shimizu and Antzelevitch, 2000; Studenik et al., 2001; Terentyev et al., 2014), yet few such studies examined [Ca2+]i transients. The lengthening of APs and [Ca2+]i transients after pharmacologically blo

• The WNT signaling pathway is a

  2018-11-08

  

  The WNT signaling pathway is a primary determinant in assigning an A/P positional identity to NPCs. This instructional cue is imparted early during NPC generation and once this identity is established, it is stable and cannot be altered through exogenous manipulation of the WNT pathway. Using a WNT

• Despite the potential for tissue specific accumulation of

  2018-11-08

  

  Despite the potential for tissue specific accumulation of dysfunctional mitochondria, MRT understandably is of considerable appeal to couples coping with a devastating mitochondrial disease as such intervention provides the only hope for a healthy child. Depending on the tissues and proportion of di

• Based on these principles we speculated that the use

  2018-11-08

  

  Based on these principles, we speculated that the use of rBC2LCN as a cargo of cytotoxic agent would provide an efficient strategy to eliminate hPSCs. Here we generated a recombinant lectin-toxin fusion protein by fusing rBC2LCN with 23 kDa of a catalytic domain of Pseudomonas aeruginosa exotoxin A

• The pluripotency of the RbiPSC line was examined by immunocy

  2018-11-08

  

  The pluripotency of the RbiPSC line was examined by immunocytochemistry staining, using Yeast Extract against human OCT4, SOX2, and SSEA4 (Fig. 1A). The in vitro spontaneous differentiation potential towards the three germ layer of RbiPSC line was demonstrated by the expression of ectodermal (βIII-

• Induced pluripotent stem cell iPSC technology Jin

  2018-11-08

  

  Induced pluripotent stem cell (iPSC) technology (Jin et al., 2012; Singh et al., 2013; Takahashi and Yamanaka, 2006; Tucker et al., 2013a,b, 2014a, 2015) makes it possible to derive cell types in vitro that would otherwise be inaccessible in living patients. This in turn allows one to evaluate the p

• We generated immortalized BMSC clones to perform detailed an

  2018-11-08

  

  We generated immortalized BMSC clones to perform detailed analysis of functional diversity in BMSC subtypes to levels not previously described. Similar immortalization techniques (e.g., hTERT combined with human papillomavirus E6/E7) have been used for the clonal analysis of BMSCs, which found OAC,

• The mechanisms involved in reprogramming somatic cells

  2018-11-08

  

  The mechanisms involved in reprogramming somatic BMS-650032 cost to iPSCs by the Yamanaka factors remain poorly understood. Because of the low efficiency and slow kinetics of most reprogramming systems, molecular events that direct somatic cells to pluripotency have been difficult to define. Recent

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